Dog Dander Allergy Test
Allergy TestsTesting KitsDog dander Allergy Test is a rapid test for the qualitative determination of Dog dander specific Immunoglobulin E (sIgE) in human serum, plasma or whole blood.
Description
Intended Use
Dog dander Allergy Test is a rapid test for the qualitative determination of Dog dander specific Immunoglobulin E (sIgE) in human serum, plasma or whole blood.
The test, in conjunction with other clinical observations, is intended to identify the patient whose allergic symptoms maybe mediated by Dog dander -specific immunoglobulin E (IgE) Type I hypersensitivity.
Principle
Dog dander Allergy Test has been designed to detect Dog dander sIgE through visual interpretation of color development in the internal strip. The membrane was immobilized with streptavidin on the test region, the conjugate pad was pre-coated with colored anti-IgE antibody colloidal gold conjugates and the sample pad was pre-coated with biotinylated Dog dander Protein. After specimens were added, the gold-conjugates move along the membrane chromatographically by capillary action and antibodies get to the test region. If sufficient Dog dander sIgE is present in the sample, it will react with biotinylated Dog dander protein in sample pad, the mixture then migrates through conjugate pad by capillary action and interact with colored anti-IgE antibody colloidal gold conjugates, form a complex. Then the complex moves to the membrane, and combine with streptavidin. As a result, a colored band will form at the test region of the membrane.
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